The experimental herbicide CGA 325'615 inhibits synthesis of crystalline cellulose and causes accumulation of non-crystalline beta-1,4-glucan associated with CesA protein.

نویسندگان

  • L Peng
  • F Xiang
  • E Roberts
  • Y Kawagoe
  • L C Greve
  • K Kreuz
  • D P Delmer
چکیده

Developing cotton (Gossypium hirsutum) fibers, cultured in vitro with their associated ovules, were used to compare the effects of two herbicides that inhibit cellulose synthesis: 2,6-dichlorobenzonitrile (DCB) and an experimental thiatriazine-based herbicide, CGA 325'615. CGA 325'615 in nanomolar concentrations or DCB in micromolar concentrations causes inhibition of synthesis of crystalline cellulose. Unlike DCB, CGA 325'615 also causes concomitant accumulation of non-crystalline beta-1,4-glucan that can be at least partially solubilized from fiber walls with ammonium oxalate. The unusual solubility of this accumulated glucan may be explained by its strong association with protein. Treatment of the glucan fraction with protease changes its size distribution and leads to precipitation of the glucan. Treatment of the glucan fraction with cellulase digests the glucan and also releases protein that has been characterized as GhCesA-1 and GhCesA-2--proteins that are believed to represent the catalytic subunit of cellulose synthase. The fact that cellulase treatment is required to release this protein indicates an extremely tight association of the glucan with the CesA proteins. In addition, CGA 325'615, but not DCB, also causes accumulation of CesA protein and a membrane-associated cellulase in the membrane fraction of fibers. In addition to the effects of CGA 325'615 on levels of both of these proteins, the level of both also shows coordinate regulation during fiber development, further suggesting they are both important for cellulose synthesis. The accumulation of non-crystalline glucan caused by CGA 325'615 mimics the phenotype of the cellulose-deficient rsw1 mutant of Arabidopsis that also accumulates an apparently similar glucan (T. Arioli, L. Peng, A.S. Betzner, J. Burn, W. Wittke, W. Herth, C. Camilleri, H. Hofte, J. Plazinski, R. Birch et al. [1998] Science 279: 717).

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The Experimental Herbicide CGA 325*615 Inhibits Synthesis of Crystalline Cellulose and Causes Accumulation of Non-Crystalline b-1,4-Glucan Associated with CesA Protein

Developing cotton (Gossypium hirsutum) fibers, cultured in vitro with their associated ovules, were used to compare the effects of two herbicides that inhibit cellulose synthesis: 2,6-dichlorobenzonitrile (DCB) and an experimental thiatriazinebased herbicide, CGA 3259615. CGA 3259615 in nanomolar concentrations or DCB in micromolar concentrations causes inhibition of synthesis of crystalline ce...

متن کامل

Dimerization of cotton fiber cellulose synthase catalytic subunits occurs via oxidation of the zinc-binding domains.

Cellulose synthase (CesA) proteins are components of CesA complexes (rosettes) and are thought to catalyze the chain elongation step in glucan polymerization. Little is understood about rosette assembly, including how CesAs interact with each other or with other components within the complexes. The first conserved region at the N terminus of plant CesA proteins contains two putative zinc finger...

متن کامل

Molecular analysis of cellulose biosynthesis in Arabidopsis.

Cellulose, an abundant, crystalline polysaccharide, is central to plant morphogenesis and to many industries. Chemical and ultrastructural analyses together with map-based cloning indicate that the RSW1 locus of Arabidopsis encodes the catalytic subunit of cellulose synthase. The cloned gene complements the rsw1 mutant whose temperature-sensitive allele is changed in one amino acid. The mutant ...

متن کامل

The effect of nanoparticles and organic acids on bacterial nano- cellulose synthesis, crystalline structure and water holding capacity

Bacterial cellulose is a biological polymer with a variety of extraordinary properties which make it a functional material for different industrial fields. This work aimed at monitoring the effects of three different organic acids and nanoparticles on the production, water holding capacity and structural characteristics of bacterial cellulose. Different concentrations of organic acids and nanop...

متن کامل

The effect of nanoparticles and organic acids on bacterial nano- cellulose synthesis, crystalline structure and water holding capacity

Bacterial cellulose is a biological polymer with a variety of extraordinary properties which make it a functional material for different industrial fields. This work aimed at monitoring the effects of three different organic acids and nanoparticles on the production, water holding capacity and structural characteristics of bacterial cellulose. Different concentrations of organic acids and nanop...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Plant physiology

دوره 126 3  شماره 

صفحات  -

تاریخ انتشار 2001